I don't think I've ever went through the effort of doing an all-encompassing list before even though villains were always one of the my most favorite things about Disney growing up. So difficult to do considering you can like a character in wildly different ways--some for comedy, some because simply for their chemistry with the protagonist, some for the dramatic power or visuals, some that you actually feel a feeling of hate for, etc. etc.
I'm sure there are a few missing--I haven't seen
Chicken Little in ages, never watched
Cars 2 or
3, and I've only seen half of
Lightyear. I debated some others, characters who are less antagonistic by the end--the Huntsman, Triton, John Silver, Georgette, the Beast, Tyler from
Turning Red, etc.--but decided to include everything for the sake of it just so I don't feel like I'm making exceptions or whatever. Same way reason that I decided to include "alternate" versions of the same characters separately since I was going to do it for the Hag v. the Queen; might as well do it for all of them. Of course, I did cheat a little and didn't include all Disney series / DTV / shorts characters, only a select few that came to mind. I couldn't bear not to have Magica de Spell, which is how I ended up going down that wormhole.
1. Ursula, the Sea Witch from
The Little Mermaid
2. Lady Tremaine, the Wicked Stepmother from
Cinderella
3. Cruella de Vil from
One Hundred and One Dalmatians
4. Hades, the God of the Underworld from
Hercules
5. Monstro from
Pinocchio ~ A lot of it is the animation. They really make you feel the weightiness of its size.
6. Yzma from
The Emperor's New Groove ~ On top of being hilarious and superbly played by Eartha Kitt, she's the only thing
visually interesting in all of TENG, imo. I don't mean "attractive" (

), just her design and the animation of it have an appealing quality.
7. Maleficent, the Dark Fairy from
Sleeping Beauty
8. Scar from
The Lion King
9. Shan-Yu, Leader of the Huns from
Mulan
10. The Rock Titan / Lythos from
Hercules ~ I decided to break them apart mostly based on design. This was always my favorite of the four, no surprise it / he comes across as the "leader" in some ways.
11. Judge Claude Frollo from
The Hunchback of Notre Dame
12. Vanessa from
The Little Mermaid
13. The Cheshire Cat from
Alice in Wonderland
14. Dragon Maleficent from
Sleeping Beauty
15. Flotsam & Jetsam from
The Little Mermaid
16. Magica de Spell from
DuckTales
17. Atropos, Lachesis, and Clotho, the Fates from
Hercules
18. Kronk from
The Emperor's New Groove
19. Madame Mim from
The Sword in the Stone
20. Dr. Facilier, the Shadow Man from
The Princess and the Frog
21. Lucifer from
Cinderella
22. The Lava Titan / Pyros from
Hercules ~ It's interesting, you look at the opening story with the Muses explaining the Titans, the Lava Titan's design looks fairly different there when compared to the ending, whereas all the others look the same. Its design seems to shift a great deal, personally my favorite was the first scene it shows him (for real) where he's more liquid and all over the place as opposed to the more solididifed flan-esque version the rest of the time (the one in the scene where he's holding Zeus back with the Ice Titan is an easy moment to go-to for what I'm referring to).
23. Orddu, Orwen, & Orgoch, the Witches of Morva from
The Black Cauldron ~ Always loved these. They definitely feel like early groundwork for when they eventually get to Ursula. Orgoch is the less interesting than the other two though.
24. The Elephant Troupe from
Dumbo ~ The Elephant Matriarch in particular is very detestable in a "realistic" way in the same fashion Frollo and Tremaine are to me.
25. Sabor from Tarzan
26. The Wind Titan / Stratos from
Hercules ~ I always think it's a shame the Wind Titan doesn't really get much chance to shine like the other three, at least, imo.
27. Cobra Jafar from
Aladdin
28. The Magic Mirror from
Snow White and the Seven Dwarfs
29. Mirage from
Aladdin (TV Series)
30. Prince Hans from
Frozen
31. Anton Ego from
Ratatouille
32. The Bowler Hat Guy / Goop & Doris from
Meet the Robinsons
33. Joanna from
The Rescuers Down Under
34. Honest John & Gideon from
Pinocchio ~ Really just Honest John, Gideon would be nearer to the bottom.
35. The Huntsman from
Snow White and the Seven Dwarfs
36. Wiggins from
Pocahontas.
37. King Triton from
The Little Mermaid ~ The character is very intimidating in almost every scene he's in, which makes the story function (since you have to understand Ariel's extreme feeling of wanting out of her life enough to think the deal doesn't seem unrealistic).
38. Namaari from
Raya and the Last Dragon
39. Zhan Tiri from
Rapunzel's Tangled Adventure
40. Mozenrath from
Aladdin (TV Series)
41. The Hag / Old Peddler-Woman from
Snow White and the Seven Dwarfs
42. Verona from
Raya and the Last Dragon
43. Georgette from
Oliver & Company
44. John Silver from
Treasure Planet
45. Sadira from
Aladdin (TV Series)
46. The Queen of Hearts from
Alice in Wonderland
47. Iago from
Aladdin ~ I like the character more in the series than the movie, but the scene where the Sultan chases him on the Carpet...
48. Kaa from
The Jungle Book
49. Helga Sinclair from
Atlantis: The Last Empire
50. Tik Tok from
Peter Pan
51. Prince John from
Robin Hood
52. Jafar, the Evil Vizier from
Aladdin
53. Te Ka from
Moana ~ The design would've been stronger in hand-drawn, but I still like it.
54. Mr. Smee from
Peter Pan
55. The Evil Queen from
Snow White and the Seven Dwarfs
56. Varian from
Rapunzel's Tangled Adventure ~ Like Morph, this is less because he's villainous (he's less interesting in villain mode actually).
57. The Hydra from
Hercules
58. Percival McLeach from
The Rescuers Down Under
59. The Horned King from
The Black Cauldron
60. Shere Khan from
The Jungle Book
61. The Bear from
The Fox and the Hound
62. The Shadows from
The Princess and the Frog
63. The Wolf from"Peter and the Wolf" of
Make Mine Music
64. Genie Jafar from
Aladdin
65. The Firebird from
Fantasia 2000
66. Chernabog from
Fantasia ~ I see right now that I put the Firebird just above Chernabog, that wasn't intentional.

There's almost four in a row that have a less "personal" quality and more "wild animal / force" concept, I think that's the why more than anything with them ending up so close. Chernabog is probably a stronger character because he dominates his short (no doubt the animation is greater), whereas the Spring Sprite dominates the one with the Firebird, but I always liked the Firebird a little more since he's more of an embodiment of natural forces. Reminds me sort of Hexxus a bit, only more serious.
67. Malcho from
Aladdin (TV Series)
68. The Ice Titan / Hydros from
Hercules ~ Always my least favorite of the Titan designs, but it's not bad or anything.
69. Cassandra from
Rapunzel's Tangled Adventure ~ Like Varian, she's more interesting outside villain mode, but the moonstone design is beautiful, no doubt.
70. Skinner from
Ratatouille
71. The Beast from
Beauty and the Beast
72. Fidget from
The Great Mouse Detective
73. Man from
Bambi
74. Kerchak from
Tarzan ~ He definitely feels the most like a "wild animal" the way Sabor does.
75. Hopper from
A Bug's Life
76. Morph from
Treasure Planet ~ So cute. So I like him in a much different way than the rest, he's only here because of being Silver's sidekick.
77. Professor Ratigan from
The Great Mouse Detective
78. Henry J. Waternoose from
Monsters, Inc.
79. Si & Am from
Lady and the Tramp
80. The Tyrannosaurus Rex from
Fantasia
81. Glut from
The Little Mermaid
82. Gaston from
Beauty and the Beast
83. Stromboli from
Pinocchio
84. Syndrome from
The Incredibles
85. Captain Hook from
Peter Pan
86. Randall Boggs from
Monsters, Inc.
87. The Wolf from
The Sword in the Stone ~ One of the more funny moments in the film, imo.
88. King Candy / Turbo from
Wreck-It Ralph
89. The Sheriff of Nottingham from
Robin Hood
90. Tyler Nguyen-Baker from
Turning Red
91. Roscoe & DeSoto from
Oliver & Company
92. Shenzi, Banzai, & Ed from
The Lion King
93. Tamatoa from
Moana
94. The Stabbington Brothers from
Tangled
95. Ronno from
Bambi
96. The Cyclops from
Hercules
97. Forte from
Beauty and the Beast: The Enchanted Christmas ~ I always liked the idea of one of the servants going insane / berserk over time because of their condition very interesting. Probably the same reason I love "Human Again" even though it does sort of bloat the movie, since the final chorus makes you feel how despairing their lives must be (the way they sing the line "Like a real human does" has a very plaintive, desperate feel to it for me).
98. The Black Cauldron from
The Black Cauldron ~ The Cauldron is often depicted like its alive--the camera panning to it, the Horned King talking to it at the end--which is why I included it.
99. Goldie OโGilt / Glittering Goldie from
DuckTales
100. Scroop from
Treasure Planet
101. The Wolves from
Beauty and the Beast
102. Drizella & Anastasia from
Cinderella
103. Mother Gothel from
Tangled
104. Governor John Ratcliffe from
Pocahontas
105. Commander Lyle Tiberius Rourke from
Atlantis: The Lost Empire
106. Mirage from
The Incredibles
107. The Leviathan from
Atlantis: The Lost Empire
108. The Headless Horseman from
The Adventures of Ichabod & Mr. Toad
109. Sir Ector & Sir Kay from
The Sword in the Stone
110. Peddler Jafar from
Aladdin
111. Morgana from
The Little Mermaid II: Return to the Sea ~ Pat Carroll is the only reason the sequel is worth watching ever.
112. Gabby Gabby from
Toy Story 4
113. The Rat from
Lady and the Tramp
114. Lawrence from
The Princess and the Frog
115. Ma Beagle from
DuckTales
116. Amos Slade from
The Fox and the Hound
117. Madame Medusa from
The Rescuers
118. Pain & Panic from
Hercules
119. The Coachman from
Pinocchio
120. Flintheart Glomgold from
DuckTales
121. The Willie Bros / Nephews from
Home On the Range
122. Mor'du from
Brave
123. Sorcerer Merlock from
DuckTales: Treasure of the Lost Lamp
124. The Flowers from
Alice in Wonderland ~ All the characters from
Wonderland are antagonists to varying degrees, I suppose. I wouldn't have included these except I always found the scene where they turn on her to be one of the more impactful ones in the film for some reason? What jerks the purple flower and the daisy in particular are.
125. Kron from
Dinosaur
126. Horace & Jasper from
One Hundred and One Dalmatians
127. Mechanicles from
Aladdin (TV Series)
128. Sir Hiss from
Robin Hood
129. Big Baby & Jolly Chimp from
Toy Story 3 ~ I only included these because of how creepy the two are in that film to me, I wanted to include them somewhere. TS3 was definitely on another level with the drama the whole movie compared to the rest in the series, even before they get to the furnace scene.
130. Bill Sykes from
Oliver & Company
131. The Druun from
Raya and the Last Dragon ~ I like the concept, but I wish the desing was better. Maybe it would work more in a hand-drawn film, whereas 3D is too limited for what they're going for with it.
132. The Jack-in-the-Box from
Fantasia 2000
133. The Evil Manta from
The Little Mermaid (TV Series) ~ The TLM series, now I think of it, had a thing for villains that were exiled (like Ursula) or sealed away--the monster awakened by the calliope, the Evil Manta, the witch in the cave from "Red." I guess they were trying to imitate Ursula to a degree (The Evil Manta uses her same colors) without actually using her, since she doesn't appear until the second season. I wonder if someone behind-the-scenes kept them from using the character purposely during the first season? The Evil Manta is really the only one that leaves much of an impression since he reoccurs; I think the first episode where he's released, he comes across very creepy actually (considering he speaks entirely different when he's trying to get Ariel to release him).
134. Alameda Slim from
Home On the Range
135. Dijon from
DuckTales
136. The Phantom Blot
137. Witch Hazel ~ Since she's often paired with the Hag and Magica de Spell, I included her.
138. Ernesto de la Cruz from
Coco
139. Mr. Snoops from
The Rescuers
140. The Carnotaurus from
Dinosaur
141. Queen Narissa from
Enchanted ~ Quite a few of the characters from here down, I find very irksome and annoying, and not so much in their capacity as villains. More because of the voice acting or dialogue or whatever not living up to what they should be in some cases, etc. I only included this one from a live-action film since she's animated. I didn't include her alternate versions, this is the best of them--the hideous dragon design being the worst.
142. Al McWhiggin from
Toy Story 2
143. Diablo from
Sleeping Beauty
144. Edgar Balthazar from
The Aristocats
145. Sid Phillips from
Toy Story
146. Chief from
The Fox and the Hound
147. Willie the Giant from
Fun and Fancy Free ~ Annoying in a stupid way.
148. Gantu from
Lilo & Stitch
149. Stinky Pete the Prospector from
Toy Story 2
150. Pete ~ Same as Willie. I've never been fond of his or Mortimer's appearances in much of anything really. I think Pete's best showing is the Prince and the Pauper film.
151. Marina del Ray from
Ariel's Beginning ~ For Sally Field, mostly.
152. Dawn Bellwether from
Zootopia
153. AUTO from
WALL E
154. LeFou from
Beauty and the Beast ~ SO ANNOYING.
155. Evil Emperor Zurg from
Toy Story
156. Evelyn Deaver / Screenslaver from
Incredibles 2
157. Dr. Calico from
Bolt ~ Even though it's a character within the universe much like Emperor Zurg, I do like the design and vibe.
158. Ercole Visconti from
Luca
159. Duke of Weselton from
Frozen
160. Tweedles Dee & Dum from
Alice in Wonderland
161. Alpha from
Up
162. Brutus & Nero from
The Rescuers
163. The Dragon from
Onward
164. Terry from
Soul
165. The Agent from
Bolt
166. The Caterpillar from
Alice in Wonderland ~ This is one character that always makes me wish someone would punch him in the face.
167. Monsieur D'Arque from
Beauty and the Beast
168. The Ringmaster from
Dumbo
169. The Beagle Boys from
DuckTales
170. Undertow from
The Little Mermaid II: Return to the Sea
171. Abis Mal from
Aladdin (TV Series)
172. The Mad Hatter & the March Hare from
Alice in Wonderland
173. Bruton from
Dinosaur
174. Creeper from
The Black Cauldron
175. Mortimer Mouse
176. Thunderclap, Downpour, & Coldfront from
The Good Dinosaur
177. Robert Callaghan from
Big Hero 6
178. Darla from
Finding Nemo
179. Scud from
Toy Story
180. Clayton from
Tarzan
181. The Fenner Brothers from
The Princess and the Frog
182. The Bensons / Ventriloquist Dummies from
Toy Story 4
183. Dean Abigail Hardscrabble from
Monsters University
184. Runeard from
Frozen II
185. Lotso from
Toy Story 3
186. Charles Muntz from
Up
187. Chick Hicks from
Cars